Evidence map›Paper›PMID 42709216›Full record

ArticleMedical microbiology and immunology2026

Identification of a novel conserved B-cell epitope in the glycoprotein of rabies virus.

Yumei Chen, Xiaohui Zhang, Yanhua Qi, Hongliang Liu, Enping Liu, Xifang Zhu, Jingming Zhou, Chao Liang, Sixuan Wu, Aiping Wang

Abstract read
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In one paragraph

Article in Medical microbiology and immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yumei ChenSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Xiaohui ZhangSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Yanhua QiSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Hongliang LiuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Enping LiuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Xifang ZhuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Jingming ZhouSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Chao LiangSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Sixuan WuSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China.
Aiping WangSchool of Life Sciences, Zhengzhou University, No. 100, Science Avenue, Zhengzhou, 450001, Henan, People's Republic of China. pingaw@126.com.ORCID https://orcid.org/0000-0003-1661-8174

Funding

General Project of Natural Science Foundation of Henan Provincial 252300421401Henan Province Major Special Projects 241110310200Major Projects of the National Nature Science Foundation of China 32192454
6 · The paper itself

Abstract

Rabies virus (RABV), a single-stranded RNA virus, invades the central nervous system and causes acute, zoonotic diseases worldwide. The glycoprotein (G) of RABV is the only surface antigen responsible for inducing potent neutralizing antibodies, making it a key target for medical countermeasures. In this study, two anti-G protein monoclonal antibodies (mAbs) were prepared using hybridoma cell technology, with their high specificity and robust reactivity validated by indirect enzyme-linked immunosorbent assay (i-ELISA), indirect immunofluorescence assay (IFA) and Western blot. Through systematic epitope mapping using a series of truncated G protein fragments, two mAbs (1G2 and 4E5) were found to recognize a minimal linear epitope, AEDFVEVHLP (amino acids 412-421) through Dot-ELISA and i-ELISA. Alanine-scanning mutagenesis further defined the core binding residues within this epitope as

Indexed as

Antigens, ViralEpitopes, B-LymphocyteGlycoproteinsRabies virusViral Envelope ProteinsAnimalsAntibodies, MonoclonalAntibodies, ViralEnzyme-Linked Immunosorbent AssayEpitope MappingFluorescent Antibody Technique, IndirectMiceMice, Inbred BALB CModels, MolecularAntibodies, MonoclonalAntibodies, ViralAntigens, ViralEpitopes, B-Lymphocyteglycoprotein G, Rabies virusGlycoproteinsViral Envelope ProteinsEpitope identificationGlycoproteinMonoclonal antibodyRabies virus

Identifiers

PMID42709216

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.