Evidence map›Paper›PMID 42709182›Full record

ArticleAnalytical and bioanalytical chemistry2026

Antigen-binding-enhanced divalent nanobody-based immunomagnetic separation coupled with ATP bioluminescence for viable Salmonella detection.

Yu Ma, Weiying Kong, Jiale Xu, Haibo Wang, Zimei Mo, Jia Wang

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yu MaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Weiying KongCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Jiale XuCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Haibo WangGuangxi-ASEAN Food Inspection Center, Nanning, 530021, Guangxi, China.
Zimei MoGuangxi-ASEAN Food Inspection Center, Nanning, 530021, Guangxi, China.
Jia WangCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. wangjia@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0001-6036-1201

Funding

National Natural Science Foundation of China 32272438
6 · The paper itself

Abstract

Current Salmonella detection methods are time-consuming or incapable of effectively discriminating viable bacteria from dead cells, restricting their practical applications in food safety monitoring. Thus, in this study, an antigen-binding-enhanced Salmonella divalent nanobody (diVHH) was optimally expressed and chemically conjugated with magnetic beads (MBs). Combined with ATP bioluminescence, a novel strategy was developed for the rapid detection of viable Salmonella. Optimization of prokaryotic expression significantly improved the diVHH yield, with an approximately 2.96-fold enhancement. Then, the diVHH-functionalized MBs achieved capture efficiencies exceeding 90% for multiple tested Salmonella serotypes. By integrating immunomagnetic separation with ATP bioluminescence, the established assay exhibited a linear detection range from 10

Indexed as

ATP bioluminescenceDivalent nanobodyMagnetic separationSalmonellaViable Salmonella detection

Identifiers

PMID42709182

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.