Evidence map›Paper›PMID 42708445›Full record

ArticleeLife2026

A simplified and highly efficient cell-free protein synthesis system for prokaryotes.

Xianshengjie Lang, Changbin Zhang, Jingxuan Lin, Zhe Zhang, Wenfei Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Xianshengjie Lang *School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.ORCID https://orcid.org/0009-0003-1857-247X
Changbin Zhang *School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.ORCID https://orcid.org/0009-0009-2006-7644
Jingxuan LinSchool of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.ORCID https://orcid.org/0009-0005-7909-0700
Zhe ZhangSchool of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.ORCID https://orcid.org/0000-0003-3616-6224
Wenfei LiSchool of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, China.ORCID https://orcid.org/0000-0002-5303-4621

Funding

Cutting Edge Development Fund of Advanced Medical Research Institute GYY2023QY01National Key Research and Development Program of China 2022YFA0807100National Natural Science Foundation of China 32171291National Natural Science Foundation of China 32371351Nature Science Foundation of Shandong Province ZR2021QC002Shandong Basic Research Special Zone YDZX2024154Shandong Basic Research Special Zone YDZX2025154Shandong Excellent Young Scientists Fund Program 2022HWYQ-025Shandong University Cheeloo Youth ProgramTaishan Scholars Program tsqnz20221104
6 · The paper itself

Abstract

Cell-free protein synthesis (CFPS) systems are a powerful platform with immense potential in fundamental research, biotechnology, and synthetic biology. Conventional prokaryotic CFPS systems, particularly those derived from

Indexed as

Escherichia coliProtein BiosynthesisBacterial LysatesCell-Free SystemBacterial Lysatesbiochemistrycell-free protein synthesischemical biologyE. coliEscherichia coli extractprotein productionsynthetic biology

Identifiers

PMID42708445
PMCPMC13553064

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.