A streamlined protocol for small-scale protoplast generation and CRISPR/Cpf1-mediated genome editing in

Jun-Ze Zheng, Szu-Chieh Huang, Wen-Ting Zeng, Ying-Hong Lin, Tao-Ho Chang

Abstract read

In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jun-Ze ZhengProgram in Plant Health Care, Academy of Circular Economy, National Chung Hsing University, Nantou 540, Taiwan.

Szu-Chieh HuangProgram in Plant Health Care, Academy of Circular Economy, National Chung Hsing University, Nantou 540, Taiwan.

Wen-Ting ZengProgram in Plant Health Care, Academy of Circular Economy, National Chung Hsing University, Nantou 540, Taiwan.

Ying-Hong LinDepartment of Plant Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.

Tao-Ho ChangProgram in Plant Health Care, Academy of Circular Economy, National Chung Hsing University, Nantou 540, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

PubMed holds no abstract for this paper.

Indexed as

CRISPR/Cpf1Fusarium oxysporumgene editingplant-microbe interactionprotoplastribonucleoprotein transformation

Identifiers

PMID42707175

DOI10.1016/j.isci.2026.117365

PMCPMC13546873

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.