ArticleeLife2026
Cortical layer 6b mediates state-dependent changes in brain activity and effects of orexin on waking and sleep.
Article in eLife, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Spatiotemporal development of sparse excitatory neuronal types within the deep mouse cortex.iScience · 2026Article
- Article
- Comprehensive mapping of the thalamic commissures in the rodent brain.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- An orexin-sensitive subpopulation of layer 6 neurons regulates cortical excitability and anxiety behaviour.Translational psychiatry · 2025Article
- The SnackerTracker: A novel home-cage monitoring device for measuring food-intake and food-seeking behaviour in mice.Wellcome open research · 2025Article
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Authors and funding
12 authors.
Funding
Abstract
One of the most distinctive features of the mammalian cerebral cortex is its laminar structure. Of all cortical layers, layer 6b (L6b) is by far the least studied, despite exhibiting direct sensitivity to orexin and having widespread connectivity, suggesting an important role in regulating cortical oscillations and brain state. We performed chronic electroencephalogram (EEG) recordings in mice in which a subset of L6b neurons was conditionally 'silenced', during undisturbed conditions, after sleep deprivation (SD), and after intracerebroventricular (ICV) administration of orexin. While the total amount of waking and sleep or the response to SD were not altered, L6b-silenced mice showed a slowing of theta frequency (6-9 Hz) during wake and rapid eye movement (REM) sleep, and a marked reduction of total EEG power, especially in non-rapid eye movement (NREM) sleep. The infusion of orexin A increased wakefulness in both genotypes, but subsequent levels of EEG slow-wave activity during NREM sleep were lower in L6b-silenced animals in the occipital derivation. In summary, these results demonstrate a role for cortical L6b in state-dependent brain oscillations and in the response to orexinergic neurotransmission. Our findings provide new insights in functions of L6b neurons and could inform the understanding of abnormal regulation of brain states in neurodevelopmental and psychiatric disorders.
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