Exploring the role of differential splicing of Pekin ducks resistant to DHAV-3 based on full-length transcriptome.

Ying Wang, Shaofei Li, Xueqin Yang, Shuaiqin Wang, Ruiping Liang, Shuisheng Hou, Xia Wang, Yunsheng Zhang

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Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Ying WangChina State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: wy_1005@nwafu.edu.cn.

Shaofei LiCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: shaofeili@nwafu.edu.cn.

Xueqin YangCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: yyyangballe@nwafu.edu.cn.

Shuaiqin WangChina State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: wangshq86@126.com.

Ruiping LiangBeijing Changping District Center for Animal Disease Prevention and Control, Beijing, 102206, China. Electronic address: ruiping80@126.com.

Shuisheng HouChina State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: houss@263.net.

Xia WangCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: xiawang@nwafu.edu.cn.

Yunsheng ZhangChina State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Inner Mongolia Key Laboratory of Duck Breeding, Inner Mongolia, Chifeng, 024207, China. Electronic address: zhangyunsheng0902@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck Hepatitis A Virus type 3 (DHAV-3) causes high mortality in Pekin ducks, and the role of post-transcriptional regulation in disease resistance remains unclear. Here, we performed Oxford Nanopore full-length transcriptome sequencing on liver samples from resistant and susceptible ducks at 0 h and 24 h after DHAV-3 infection. Only one differentially expressed gene (DEG) and four differentially expressed transcripts (DETs) were identified pre-infection (0 h), whereas post-infection (24 h) we detected 2,539 DEGs and 2,909 DETs, indicating strong group-specific expression divergence. Functional enrichment revealed immune-related and metabolic pathway dysregulation in susceptible ducks, while resistant ducks maintained homeostasis. BulkSignalR identified an ECM-Integrin-JAK/Src module unique to the resistant group. Alternative splicing (AS) events increased from 16 pre-infection to 310 post-infection, enriched in immune and signaling pathways. Key immune genes showed isoform switching; in susceptible ducks, SELENOP transcripts lacked multiple selenocysteine sites, and a truncated PRPF8 isoform was highly expressed, while high DHX58 expression may suppress RIG-I activation. These findings highlight the critical role of post-transcriptional regulation in antiviral immunity and provide potential targets for improvement of disease resistance.

Indexed as

Alternative splicingDHAV-3DuckFull-length transcriptome sequencingIsoform switch

Identifiers

PMID42705198

DOI10.1016/j.psj.2026.107640

PMCPMC13572196

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