Evidence map›Paper›PMID 42704481›Full record

ArticleMikrochimica acta2026

A ultrasensitive immunoassay based on weakly charged AuNPs orientated antibody for triazophos detection in vegetables.

Qinghuan Wu, Qijun Wang, A M Abd El-Aty, Xing Zhang, Guangyang Liu, Hui Li, Xinyan Liu, Lin Qin, Xiaodong Huang, Donghui Xu and 1 more

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Qinghuan Wu *State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
Qijun Wang *College of Horticulture and Landscape Architecture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, 400715, P. R. China.
A M Abd El-AtyDepartment of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Xing ZhangState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
Guangyang LiuState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
Hui LiState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
Xinyan LiuState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
Lin QinState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China.
Xiaodong HuangState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China. huangxiaodong@caas.cn.
Donghui XuState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China. xudonghui@caas.cn.
Ge ChenState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Key Laboratory of Vegetables Quality and Safety Control, Ministry of Agriculture and Rural Affairs of China, Ministry of Agriculture Vegetable Product Quality Safety Risk Assessment Laboratory, Ministry of Agriculture and Rural Affairs of China, Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China. chenge@caas.cn.

Funding

China Agriculture Research System of MOF and MARA CARS-23-E03National Key Research and Development Program of China 2022YFF0606800National Natural Science Foundation of China 32302207, 32402242The Natural Science Foundation of Chongqing CSTB2022NSCQ-MSX1461
6 · The paper itself

Abstract

Immunoassays based on gold nanoparticles (AuNPs) often suffer from limited sensitivity. Herein, we synthesized weakly charged AuNPs using ascorbic acid and establish a competitive immunoassay for the detection of triazophos. The weak electronegativity of these AuNPs reduces electrostatic repulsion during antibody adsorption, leading to increased antibody loading and favorable antibody orientation, thereby enhancing detection sensitivity. The triazophos antibody and horseradish peroxidase (HRP) were electrostatically adsorbed onto the AuNP surface to form an antibody-AuNP-enzyme probe complex. In the immunoassay, triazophos in the sample competes with the coated antigen for binding to the Ab@AuNPs@HRP probe, and quantification is achieved through an HRP-catalyzed colorimetric reaction. The proposed method demonstrated a linear range of 1.08-22.48 µg/L, an IC

Indexed as

AntibodiesGoldMetal NanoparticlesOrganothiophosphatesTriazolesVegetablesColorimetryFood ContaminationHorseradish PeroxidaseImmunoassayLimit of DetectionAntibodiesGoldHorseradish PeroxidaseOrganothiophosphatesTriazolestriazophosAb@AuNPs@HRPAuNPsCompetitive immunoassayHRPTriazophos detection

Identifiers

PMID42704481

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.