ArticleInternational journal of fertility & sterility2026
Gene Expression Dynamics and Network Analysis Reveal Key Molecular Pathways in Spermatogenic Progression from Spermatogonial Stem Cells to Round Spermatids.
Article in International journal of fertility & sterility, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Special Issue "Advances in Spermatogenesis and Male Infertility".International journal of molecular sciences · 2026Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: Spermatogenesis is a tightly regulated process that involves an orchestrated transcriptional and translational event across the seminiferous epithelium. While individual regulators of this process have been studied, the network-level dynamics underlying the transition from spermatogonial stem cells (SSCs) to round spermatids (RSs) remain poorly understood. The dynamic molecular transitions between SSCs and RSs have not been fully characterised, particularly at the network level. Therefore, this study aimed to characterise the molecular and network-level dynamics underlying the transition from SSCs to RSs by integrating transcriptomic profiling, protein-protein interaction network analysis, functional enrichment, and immunohistochemical validation. Materials and Methods: In this experimental study, we evaluated the gene expression dynamics from SSCs to RSs. We analysed publicly available microarray datasets (GEO) comprising four biological replicates for RSs and three biological replicates for SSCs. Differentially expressed genes (DEGs) were identified and mapped onto proteinprotein interaction (PPI) networks, followed by prioritisation of central regulators. Functional enrichment analysis was performed to characterise biological pathways. Immunohistochemistry (IHC) for SOX9, n-MYC, VASA, SOX2, and DAZL was conducted to examine spatial and developmental protein expressions across the seminiferous tubules. Results: A set of 3598 DEGs was identified between SSCs and RSs. This analysis highlighted central genes, Conclusion: This study used transcriptomic profiling with network analysis and molecular experiments to reveal novel regulatory connections that underlie the SSC-RS transition. These findings provide a systems-level framework for understanding male germline development and may facilitate future research on infertility and reproductive health.
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Registered trials
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