Evidence map›Paper›PMID 42702597›Full record

ArticleNature communications2026

Uncovering the signaling networks of disseminated glioblastoma cells in vivo with INSIGHT.

Ryuhjin Ahn, Alicia D D'Souza, Lawrence Long, Yufei Cui, Jennifer Gantchev, Gerard Baquer, Danielle Burgenske, Katrina K Bakken, Lauren L Ott, Brett L Carlson and 16 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Critical role of cell competition in gliomagenesis.bioRxiv : the preprint server for biology · 2026
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

26 authors.

Ryuhjin AhnDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA. ryuhjin.ahn@mcgill.ca.ORCID http://orcid.org/0000-0001-9788-4401
Alicia D D'SouzaDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Lawrence LongDepartment of Biological Engineering, MIT, Cambridge, MA, USA.ORCID http://orcid.org/0009-0003-8923-5518
Yufei CuiDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-3014-813X
Jennifer GantchevDepartment of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Gerard BaquerDepartment of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-4433-4972
Danielle BurgenskeMayo Clinic, Rochester, MN, USA.
Katrina K BakkenMayo Clinic, Rochester, MN, USA.
Lauren L OttMayo Clinic, Rochester, MN, USA.
Brett L CarlsonMayo Clinic, Rochester, MN, USA.
Grace ZhouMIT, Cambridge, MA, USA.
Ishwar N KohaleDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Charles A WhittakerDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-2400-0094
Heidi TempleDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.ORCID http://orcid.org/0009-0004-7437-7350
Tomer M Yaron-BarirColumbia University Vagelos College of Physicians and Surgeons, New York, NY, USA.ORCID http://orcid.org/0000-0001-6574-7314
Jeffrey WyckoffDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Terry C BurnsMayo Clinic, Rochester, MN, USA.ORCID http://orcid.org/0000-0001-7633-3045
Rachael A VaubelMayo Clinic, Rochester, MN, USA.ORCID http://orcid.org/0000-0001-8842-5975
Cameron T FlowerDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-9632-9913
Wei HuangPreclinical Imaging and Testing Facility of the Swanson Biotechnology Center, David H. Koch Institute for Integrative Cancer Research, MIT, Cambridge, MA, USA.ORCID http://orcid.org/0009-0004-1965-5928
Ann TumaMayo Clinic, Rochester, MN, USA.
Jared L JohnsonDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0003-1802-6527
Nathalie Y R AgarDepartment of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Josie Ursini-SiegelDivision of Experimental Medicine, McGill University, Montreal, QC, Canada.ORCID http://orcid.org/0000-0003-3242-3617
Jann N SarkariaMayo Clinic, Rochester, MN, USA.ORCID http://orcid.org/0000-0001-7489-4885
Forest M WhiteDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA. fwhite@mit.edu.ORCID http://orcid.org/0000-0002-1545-1651

Funding

VIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Jacqueline A. Lees · 1985 to 2026
$93.9M
Quantitative systems biology of glioblastoma cells and their interactions with the neuronal and immunological milieuU54CA283114 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Forest M White · 2023 to 2026
$9.7M
NCI NIH HHS P30 CA014051NCI NIH HHS U54 CA283114U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) P30 CA14051U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) U54 CA283114
6 · The paper itself

Abstract

Dysregulation of intracellular signaling networks underpins cancer. Yet, resolving signaling networks within distinct or rare cell types in cancer in vivo has been unattainable. Here we develop INSIGHT by integrating cell sorting with mass spectrometry to enable quantitative phosphoproteomics and proteomics of discrete cell types from fixed tissues. Using INSIGHT, we map the signaling network within disseminating glioblastoma cells from patient-derived xenografts implanted in mice. Disseminating tumor cells undergo a proteome-wide shift from proliferative to mesenchymal, neural progenitor-like cell states. In parallel, signaling network and global kinase activity are rewired, transitioning from cell cycle-associated circuitries to those governing synaptic function, neuronal migration, and ion channel activity. Changes begin at the tumor margin and persist in distant brain parenchyma. Hornerin and phosphorylation of Ca²⁺-permeable GluA2 at Y876 were identified as mediators of glioblastoma progression. INSIGHT enables systems-level dissection of cell-type-specific signaling circuitries in vivo across wide range of biological systems.

Indexed as

Brain NeoplasmsGlioblastomaSignal TransductionAnimalsCell Line, TumorHumansMass SpectrometryMicePhosphorylationProteomeProteomicsReceptors, AMPAProteomeReceptors, AMPA

Identifiers

PMID42702597
PMCPMC13547291

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.