Evidence map›Paper›PMID 42701171›Full record

ArticleMycopathologia2026

Diagnostic Efficacy of Calcofluor White Staining Versus PAS and GMS Staining for Human Sporotrichosis in FFPE Specimens.

Lina Li, Lijie Zhang, Shiyou Du, Shuai Zhang, Shoumin Zhang, Hongwei Liu, Jianguo Li, Yuping Ran

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In one paragraph

Article in Mycopathologia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Lina LiDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Lijie ZhangDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Shiyou DuDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Shuai ZhangDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Shoumin ZhangDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Hongwei LiuDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Jianguo LiDepartment of Dermatovenereology, Henan Provincial People's Hospital (People's Hospital of Zhengzhou University), Zhengzhou, China.
Yuping RanDepartment of Dermatovenereology, West China Hospital, Sichuan University, Chengdu, China. ranyuping@vip.sina.com.

Funding

Dermatophytosis Fluorescence Examination and Fungal Culture Technology Promotion Project in Primary Care Hospitals SYJS2020037
6 · The paper itself

Abstract

objectiveThis study aimed to evaluate the diagnostic efficacy of calcofluor white (CFW) staining for sporotrichosis using archival formalin-fixed paraffin-embedded (FFPE) specimens, compared with conventional histochemical stains.

methodsA total of 75 FFPE specimens collected between 2010 and 2023 from patients with culture- or sequencing-confirmed sporotrichosis cases were enrolled, with 70 specimens from non-fungal infection cases using as controls. Serial tissue sections were stained with CFW, periodic acid-Schiff (PAS), and Gomori methenamine silver (GMS) staining, respectively. The detection rates, diagnostic accuracy metrics, and storage duration effects were assessed.

resultsCFW staining yielded a fungal detection rate of 78.6% (59/75), which was significantly higher than those of PAS (38.6%, 29/75) and GMS (28.0%, 21/75) (χ

conclusionsFor archival FEPE specimens, CFW staining exhibits superior sensitivity and excellent specificity for sporotrichosis detection, which enables rapid diagnosis and effectively reduces the risk of misdiagnosis. Considering its technical simple operation, long-term stability, and being compatible with conventional histological examination, CFW staining serves as a valuable adjunctive diagnostic tool, particularly in sporotrichosis- endemic regions. Further prospective multicenter studies were required to validate its clinical application value.

Indexed as

BenzenesulfonatesSporotrichosisStaining and LabelingAdultFemaleHumansMaleMiddle AgedParaffin EmbeddingPeriodic Acid-Schiff ReactionSensitivity and SpecificityBenzenesulfonatesC.I. Fluorescent Brightening Agent 28Calcofluor white stainingGomori methenamine silver stainingInvasive fungal infectionPeriodic acid-Schiff stainingSporotrichosis

Identifiers

PMID42701171

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