Evidence map›Paper›PMID 42700503›Full record

ArticlePoultry science2026

Transcriptomic regulation of sequential eggshell mineralization in laying hens: Molecular mechanism underlying eggshell quality and production performance implications.

Xiaofan Yu, Xinye Zhang, Xing Chen, Yalan Zhang, Dehe Wang, Mengyuan Wang, Yuhang Peng, Xiaolong Ma, Yuhui Xie, Zhonghua Ning and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xiaofan YuNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; College of Agriculture, Enshi Polytechnic, Enshi 445000, China.
Xinye ZhangNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Xing ChenNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yalan ZhangNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Dehe WangCollege of Animal Science and Technology, Hebei Agricultural University, Baoding Hebei 071001, China.
Mengyuan WangNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yuhang PengNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Xiaolong MaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Yuhui XieNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Zhonghua NingNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: ningzhh@cau.edu.cn.
Lujiang QuNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. Electronic address: quluj@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eggshell quality is a key economic trait in commercial layer production, which profoundly affects economic profits, flock elimination rate, and feeding management. Frequent occurrence of cracked, thin-shelled eggs has long plagued intensive laying hen farming. This study aimed to dissect the molecular mechanisms and regulatory networks underlying staged eggshell mineralization across the pre-mineralization, initial mineralization, and rapid mineralization phases in laying hens, and explore their links to eggshell quality and shell defect in production. By integrating RNA-sequencing, temporal expression profiling and WGCNA, we screened core functional genes and signaling pathways in uterus that governing the variation of eggshell thickness. In pre-mineralization stage, high expression of Ovalbumin (OVAL) may inhibit premature calcium carbonate crystallization and avoid abnormal shell development. During rapid mineralization, genes encoding eggshell matrix proteins, ATPase, TRP (transient receptor potential), SLC (Solute carrier) and collagen family members coordinately maintained uterine cellular calcium homeostasis, sustained calcium supply for eggshell deposition, and ultimately determined eggshell thickness and strength. Meanwhile, calcium concentration in uterine fluid increased gradually along with mineralization and remained stable throughout rapid mineralization phase, providing a steady calcium supply basis for preventing eggshell defects in commercial laying hen production. Findings of this study provided targets and theoretical support for the breeding of laying hen breeds, the regulation of nutritional management, and the optimization of farming environment management.

Indexed as

CalcificationCalcium transportChickenEggshell matrix proteinUterus

Identifiers

PMID42700503
PMCPMC13572529

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.