Evidence map›Paper›PMID 42700292›Full record

ArticleMolecular biology reports2026

Mitogenomic and phylogenomic analyses identify a cohesive Western Atlantic lineage within the Narcine complex (Torpediniformes: Narcinidae).

Paola Palacios-Barreto, Beatriz Rochitti Boza, Vanessa Paes da Cruz, Bruno Lopes da Silva Ferrette, Claudio Oliveira, Matheus Marcos Rotundo, Nadia Sandoval-Laurrabaquio-Alvarado, Douglas H Adams, Píndaro Díaz-Jaimes

Abstract read
In one paragraph

Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Paola Palacios-BarretoFundación Colombiana Para La Investigación y Conservación de Tiburones y Rayas, SQUALUS, Cali, Colombia.ORCID http://orcid.org/0000-0003-2441-6791
Beatriz Rochitti BozaLaboratório de Biologia E Genética de Peixes, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-8641-2174
Vanessa Paes da CruzLaboratório de Biologia E Genética de Peixes, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-2450-8701
Bruno Lopes da Silva FerretteLaboratório de Biologia E Genética de Peixes, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-3108-9867
Claudio OliveiraLaboratório de Biologia E Genética de Peixes, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Botucatu, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-7010-8880
Matheus Marcos RotundoAcervo Zoológico da Universidade de Santa Cecília, Santos, São Paulo, Brazil.
Nadia Sandoval-Laurrabaquio-AlvaradoEl Colegio de La Frontera Sur (ECOSUR), Campeche, México.
Douglas H AdamsFlorida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute, Indian River Field Laboratory, Melbourne, FL, USA.
Píndaro Díaz-JaimesUnidad Académica de Ecología y Biodiversidad Acuática, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad de Mexico, México. pindaro@cmarl.unam.mx.ORCID https://orcid.org/0000-0002-3230-3538

Funding

Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT), UNAM IN207425
6 · The paper itself

Abstract

backgroundAccurate species delimitation within electric rays of the genus Narcine has been hindered by overlapping morphological characters and limited molecular resolution in previous single-locus studies. This study aims to evaluate phylogenetic relationships and species boundaries within the Narcine species complex across the Western Atlantic using complete mitochondrial genomes. METHODS AND

resultsSeven complete mitogenomes were newly assembled from individuals representing distinct morphotypes sampled across geographically widespread Western Atlantic localities and analyzed together with publicly available reference sequences. Mitochondrial protein-coding genes (PCGs) were examined using concatenated nucleotide and amino acid datasets under partitioned maximum-likelihood frameworks. Both approaches recovered highly congruent topologies, consistently supporting a single, well-defined western Atlantic mitochondrial lineage with low internal divergence (0.04-2.13%). Species delimitation analyses based on multiple methods yielded partially congruent results but consistently identified a dominant lineage encompassing all Atlantic samples. In contrast, two Colombian reference mitogenomes formed a separate and highly divergent lineage relative to the Atlantic group, despite showing moderate divergence between them. Comparative mitogenomic analyses revealed conserved genome organization, nucleotide composition bias, codon usage, and transfer RNA (tRNA) structures. All PCGs evolved under strong purifying selection, with Ka/Ks ratios well below unity.

conclusionsThese results support mitochondrial genetic continuity across the Western Atlantic Narcine populations and do not provide mitochondrial evidence for multiple evolutionary lineages within the Western Atlantic. The marked mitochondrial divergence of Colombian reference mitogenomes highlights potential issues in sequence attribution and underscores the importance of data curation. Overall, complete mitochondrial genomes provide a robust framework for species delimitation and future integrative taxonomic assessments within Narcine.

Indexed as

Genome, MitochondrialAnimalsAtlantic OceanDNA, MitochondrialEvolution, MolecularPhylogenyRNA, TransferDNA, MitochondrialRNA, TransferComplete mitochondrialElectric rayPhylogenetic relationshipsSpecies delimitation

Identifiers

PMID42700292
PMCPMC13546158

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.