Evidence map›Paper›PMID 42700284›Full record

ArticleVeterinary research communications2026

Molecular investigation of coronavirus and Leptospira spp. in bats (Chiroptera, Mammalia) from Brazil.

Raquel Cuba Gaspar, Benedito Donizete Menozzi, Anna Luisa Pizzaia Henrique, Gismelli Cristiane Angeluci, Nycolas Octavio Ribeiro Carvalho, Katia Jaggi, Gabrielle Dos Santos Rocha, Tayná Giovana Alaniz, Luiz Gustavo Bentim Góes, Márcia Marinho and 2 more

Abstract read
In one paragraph

Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Raquel Cuba GasparDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Benedito Donizete MenozziDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Anna Luisa Pizzaia HenriqueDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Gismelli Cristiane AngeluciDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Nycolas Octavio Ribeiro CarvalhoDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Katia JaggiDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Gabrielle Dos Santos RochaInstitut Pasteur of São Paulo, São Paulo, Brazil.
Tayná Giovana AlanizDepartment of Production and Animal Health, São Paulo State University, Araçatuba, Brazil.
Luiz Gustavo Bentim GóesInstitut Pasteur of São Paulo, São Paulo, Brazil.
Márcia MarinhoDepartment of Production and Animal Health, São Paulo State University, Araçatuba, Brazil.
Helio LangoniDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil.
Felipe FornazariDepartment of Animal Production and Preventive Veterinary Medicine, São Paulo State University, Botucatu, São Paulo, Brazil. f.fornazari@unesp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The emergence of zoonotic diseases highlights the need for continuous surveillance of wildlife, particularly bats, known as natural reservoirs of several pathogens. This study aimed to investigate the presence of coronaviruses (CoV) and Leptospira spp. in non-hematophagous bats from the northwestern region of São Paulo State, Brazil, between 2019 and 2024. Samples of intestine and kidney from 98 specimens from 18 species belonging to three families (Molossidae, Phyllostomidae, and Vespertilionidae) were collected in 8 municipalities of the Araçatuba Intermediate Geographic Region. Coronaviruses detection was performed by pan-CoV nested RT-PCR, targeting fragments of 602 and 440 bp of the RNA-dependent RNA polymerase (RdRp) gene. Investigation of Leptospira spp. was conducted by real-time PCR, aiming the amplification of a fragment of 331 bp of the 16 S rRNA gene. The analyses yielded no amplification in any of the intestinal and renal samples. Due to geographic bias and the underrepresentation of phyllostomids - a central taxon in CoV epidemiology in Brazil -, the lack of detection reflects sampling limitations rather than the real absence of these pathogens in the local fauna. Continuous surveillance, integrated with comprehensive and standardized sampling in this under-studied region, remains essential for the early detection of emerging zoonotic pathogens and for characterizing local host-pathogen ecology.

Indexed as

ChiropteraCoronavirusCoronavirus InfectionsLeptospiraLeptospirosisAnimalsBrazilKidneyPhylogenybat-borne pathogensChiropteraCoronavirusLeptospiraMolecular surveillance

Identifiers

PMID42700284
PMCPMC13546309

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.