Evidence map›Paper›PMID 42699109›Full record

ArticleMolecular therapy. Nucleic acids2026

Simultaneous quantitative detection of multiple low-frequency variants by high-dynamic-range capillary electrophoresis.

Yoshihiko Hagiwara, Nobue Tamamura, Noriyuki Sumida, Yusuke Ono, Kenji Takahashi, Yanpeng Sun, Kazuya Koyama, Yu Ohtaki, Tetsuhiro Okada, Hidemasa Kawabata and 10 more

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In one paragraph

Article in Molecular therapy. Nucleic acids, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Yoshihiko HagiwaraHitachi High-Tech Corporation, Tokyo 105-6409, Japan.
Nobue TamamuraDepartment of Advanced Genomic Community Healthcare, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Noriyuki SumidaHitachi High-Tech Corporation, Tokyo 105-6409, Japan.
Yusuke OnoDepartment of Advanced Genomic Community Healthcare, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Kenji TakahashiDepartment of Advanced Genomic Community Healthcare, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Yanpeng SunDepartment of Advanced Genomic Community Healthcare, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Kazuya KoyamaDivision of Gastroenterology, Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Yu OhtakiDivision of Gastroenterology, Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Tetsuhiro OkadaDivision of Gastroenterology, Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Hidemasa KawabataDivision of Gastroenterology, Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Chiho MaedaInstitute of Biomedical Research, Sapporo-Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan.
Miyuki MoriInstitute of Biomedical Research, Sapporo-Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan.
Chiemi MatsumotoInstitute of Biomedical Research, Sapporo-Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan.
Mayumi SuzukiInstitute of Biomedical Research, Sapporo-Higashi Tokushukai Hospital, Sapporo, Hokkaido 065-0033, Japan.
Shin-Ichi ChibaCenter for Advanced Research and Education, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Mishie TaninoDepartment of Diagnostic Pathology, Asahikawa Medical University Hospital, Asahikawa, Hokkaido 078-8510, Japan.
Kenzui TaniueDivision of Gastroenterology, Department of Medicine, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.
Hirokazu KatoHitachi High-Tech Corporation, Tokyo 105-6409, Japan.
Motohiro YamazakiHitachi High-Tech Corporation, Tokyo 105-6409, Japan.
Yusuke MizukamiDepartment of Advanced Genomic Community Healthcare, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sensitive and quantitative detection of low-frequency variants across multiple loci is critical for nucleic acid-based diagnostics, yet clinical implementation requires a balance among sensitivity, multiplexing capacity, cost, and operational simplicity. We previously developed a high-dynamic-range capillary electrophoresis system capable of detecting variants at allele frequencies below 1%; however, its application was limited to single-locus analysis. Here, we expanded this platform to multiplex detection by incorporating mobility-shift strategies into the assay design. This approach enabled simultaneous analysis of 15 hotspot variants across three clinically relevant loci:

Indexed as

cancercapillary electrophoresisdynamic rangegenetic analysisHiDymolecular diagnosticsMT: clinical applicationsmultiplex shifted termination assaypancreatic diseasequantitative analysis

Identifiers

PMID42699109
PMCPMC13542593

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.