ArticleJDS communications2026
Measuring cytokines in quarter milk samples with a fluorescent bead-based multiplex assay.
Article in JDS communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Mastitis is a highly prevalent, costly disease affecting well-being and productivity in dairy cows. Management of intramammary infections typically relies on aerobic culture for identification of a causative pathogen. Cytokines regulate inflammatory processes in the infected quarter. Their production and secretion patterns are influenced by the invading pathogen and by the host's immune system. A previously validated fluorescent bead-based multiplex assay was used for the quantification of bovine IL-10, TNF-α, and IFN-γ. Our goal was to demonstrate that this assay can be used for milk samples and to measure concentrations of the 3 analytes in quarter milk samples with different bacteriological culture results. We enrolled quarter milk samples that were submitted for routine mastitis diagnostics by farm personnel from commercial dairies (n = 17) in central New York. We selected samples identified with major mastitis pathogens, including coliform bacteria (n = 51; termed the coliform group, comprising
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