ArticleMicrobiology spectrum2026
Comparative performance of portable DNA extraction protocols and bioinformatics workflows for rapid detection of gram-negative bacteria and antimicrobial resistance using Oxford Nanopore sequencing.
Louis Kyei-Tuffuor, Samuel Kekeli Agordzo, Angela Krobea Asante, Davina Serwaa Boateng, Winifred Nana Serwaa Adjei, Venus Nana Boakyewaa Frimpong, Rita Nartey, Francis Agyemang-Yeboah, Rea Maja Kobialka, Ahmed Abd El Wahed and 4 more
Abstract readComparative Study
In one paragraphArticle in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
14 authors.
Louis Kyei-TuffuorKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.ORCID 0000-0001-5607-3215 Samuel Kekeli AgordzoKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Angela Krobea AsanteKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Davina Serwaa BoatengKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Winifred Nana Serwaa AdjeiKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Venus Nana Boakyewaa FrimpongKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Rita NarteyKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Francis Agyemang-YeboahDepartment of Molecular Medicine, School of Medical Sciences (SMS), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Rea Maja KobialkaInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.ORCID 0009-0007-5114-1910 Ahmed Abd El WahedInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.ORCID 0000-0003-3347-6075 Uwe TruyenInstitute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.ORCID 0000-0003-1275-9586 Yaw Ampem AmoakoKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Richard Odame PhillipsKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Michael FrimpongKumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.ORCID 0000-0003-1901-6793 Funding
German Federal Ministry of Research, Technology and Space 01KA2218B
6 · The paper itselfAbstract
Oxford Nanopore Technology (ONT) enables rapid, portable pathogen identification and antimicrobial resistance (AMR) detection, but the reliability of downstream genomic analyses is highly dependent on DNA extraction quality, particularly in resource-limited settings. This study comparatively evaluated four portable bacterial DNA extraction protocols derived from three commercial kits to determine their impact on nanopore sequencing performance, bioinformatics workflow completion, and field deployability. Six gram-negative bacterial isolates (
Indexed as
Computational BiologyDNA, BacterialDrug Resistance, BacterialGram-Negative BacteriaNanopore SequencingAnti-Bacterial AgentsSequence Analysis, DNAWorkflowAnti-Bacterial AgentsDNA, Bacterialantimicrobial resistancebioinformatics workflowDNA extractionnanopore sequencingpathogen identificationportable diagnosticsresource-limited settingswhole-genome sequencing
Identifiers
PMID42695686
PMCPMC13689490
What OpenQuestion holds
Textmetadata
Read underepoch 390