Pharmacokinetic and Tissue Distribution Analysis of Scutellarin and Iso-Scutellarin From Breviscapine Injection in Rats by a Validated UPLC-MS/MS Method.

Min Wen, Lijuan Fan, Lipan Yang, Qi Zhang, Xiaobo Lv

Abstract read

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Article in Biomedical chromatography : BMC, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Min WenSchool of Ethnic Medicine, Yunnan Minzu University, Kunming, China.ORCID https://orcid.org/0000-0002-6469-676X

Lijuan FanKunming Longjin Pharmaceutical Co. Ltd, Kunming, China.

Lipan YangCollege of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, China.

Qi ZhangSchool of Ethnic Medicine, Yunnan Minzu University, Kunming, China.

Xiaobo LvKunming Longjin Pharmaceutical Co. Ltd, Kunming, China.

Funding

Key Laboratory of Chemistry in Ethnic Medicinal Resources MZY2606National Natural Science Foundation of China 81960654State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University

6 · The paper itself

Abstract

Breviscapine injection (BI), a standardized botanical drug for cardiovascular and cerebrovascular diseases, lacks adequate characterization of its pharmacokinetics and tissue distribution. A UPLC-MS/MS method was developed and validated for simultaneous quantification of scutellarin and its metabolite iso-scutellarin in rat plasma and tissues after intravenous BI administration. Sample preparation used methanol protein precipitation, with detection via positive electrospray ionization MRM. Validation followed the Chinese Pharmacopoeia and bioanalytical PK guidance. The assay showed LLQs of 10.0 ng/mL (scutellarin) and 5.0 ng/mL (iso-scutellarin), good linearity (r

Indexed as

ApigeninFlavonoidsGlucuronatesLiquid Chromatography-Mass SpectrometryTandem Mass SpectrometryAnimalsChromatography, High Pressure LiquidLimit of DetectionLinear ModelsMaleRatsRats, Sprague-DawleyReproducibility of ResultsTissue DistributionApigeninbreviscapineFlavonoidsGlucuronatesscutellarinbreviscapine injectionpharmacokineticsquantificationtissue distributionUPLC‐MS/MS

Identifiers

PMID42695236

DOI10.1002/bmc.70608

PMCPMC13543043

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.