ArticleFrontiers in nutrition2026
Ultrasound-assisted deep eutectic solvent extraction of polyphenols from peach leaves and their compositional characterization.
Article in Frontiers in nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This work reports the extraction of polyphenols from peach leaves via ultrasound-assisted deep eutectic solvent extraction (UAE-DES) coupled with subsequent resin-based purification, along with comprehensive characterization of the resulting phenolic profile. Screening of various DES formulations identified the choline chloride-lactic acid system as the most effective for this purpose. Based on single-factor experiments and Box-Behnken response surface design, the optimal UAE-DES extraction parameters were determined as follows: liquid-to-solid ratio of 26.61 mL/g, ultrasonic temperature of 40 °C, choline chloride-to-lactic acid molar ratio of 1:3.715, water content of 20%, and sonication time of 10 min. Under these optimal settings, the total phenolic content (TPC) attained 16.79 mg gallic acid equivalents (GAE)/g, closely approaching the model-predicted value of 16.92 mg GAE/g. The crude extract was subsequently purified using AB-8 macroporous resin, and Fourier transform infrared spectroscopy (FTIR) analysis confirmed the presence of functional groups characteristic of phenolic and flavonoid structures. UPLC-MS/MS profiling further identified 25 distinct phenolic compounds, with flavonol glycosides representing the dominant category, accounting for 91.07% of the total quantified polyphenols. Among these, quercetin-3-O-glucoside (50.75%), kaempferol-3-O-glucoside (20.71%), and rutin (19.61%) were identified as the most abundant components.
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