ArticleFrontiers in microbiology2026
Stool microbiota variations along the adenoma-colorectal carcinoma sequence - robustness of disease-associated microbial features.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Colorectal cancer (CRC) has been linked with gut microbiota dysbiosis, thereby fostering the discovery of disease-associated microbial signatures. However, the use of covariate-adjusted methods accounting for confounders effects on microbiome shifts is far from being a gold standard approach, leading to spurious microbial-disease associations. This study aimed to characterize stool microbiota alterations along the adenoma-carcinoma sequence in a Portuguese cohort, and assess the robustness of candidate microbial features. Methods: Stool samples from healthy individuals (HC), adenoma patients (AP), and CRC patients at early (SI/II) and advanced (SIII/IV) stages, were analyzed using 16S rRNA gene amplicon sequencing. Microbial features' profiles were compared with publicly available datasets from France, Ireland, Italy, and USA/Canada. Differential abundance (DA) analysis was conducted through multiple DA models (ANCOM-BC2, fastANCOM, MaAsLin2, limma voom, and LEfSe) and statistical approaches, including covariate (age, sex, and body mass index (BMI)) adjustment, prevalence filtering, and analysis of matched subsets. Results and discussion: Among the altered taxa along de adenoma-carcinoma sequence, Conclusion: Overall, our results strengthen the importance of covariate control towards the identification of potentially robust and reproducible microbial markers of CRC onset and progression.
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