Association between animal welfare indicators and mastitis pathogens in bulk tank milk: the reality of small- and medium-scale dairy cattle farms in Italy.

Introduction: Animal welfare is increasingly recognized as a key determinant of dairy herd health, yet its relationship with the presence of mastitis pathogens at the herd level remains insufficiently explored. This study aimed to evaluate the association between animal welfare indicators and the presence of major mastitis pathogens in bulk tank milk (BTM) from dairy cattle herds in the Aosta Valley, Italy, while also characterizing microbial profiles using bacteriological culture and polymerase chain reaction (PCR). Methods: A broad microbiological screening was performed on unique BTM samples from 490 individual farms. Concurrently, a subset of 355 small- and medium-scale dairy cattle farms underwent detailed animal welfare assessments using the standardized ClassyFarm protocol. Data were collected by experienced assessors in northern Italy in 2022, with each farm visited once. Results: The median lactating herd size was 33 cows (IQR: 57-18) and included Aosta Red Pied and Aosta Black Pied cattle breeds. All farms were tie-stall in the Aosta Valley, where herds are grazed outdoors for most of the year and kept indoors in tied-stalls during winter. BC identified at least one pathogen in 73.5% of samples, with most positive samples exhibiting low diversity, predominantly single (48.6%) or dual-species (41.1%) profiles. Discussion: These findings provide evidence that improved animal welfare is associated with a lower likelihood of major mastitis pathogens in BTM.