Evidence map›Paper›PMID 42689863›Full record

ArticleCell biology international2026

ROS-Dependent GSDMD Activation Drives Intestinal Pyroptosis in Neonatal Necrotizing Enterocolitis (NEC).

Cuilian Ye, Jia Luo, Xinli Liu, Yue Ma, Xinyi Yang, Dandan Mo, Qin Deng, Xionghui Ding, Chunbao Guo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell biology international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Cuilian YeSchool of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, China.
Jia LuoSchool of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, China.
Xinli LiuSchool of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, China.
Yue MaDepartment of Pediatrics, Chongqing Health Center for Women and Children, Chongqing, China.
Xinyi YangSchool of Pharmacy, Chongqing Medical University, Chongqing, China.
Dandan MoDepartment of Pediatrics, Chongqing Health Center for Women and Children, Chongqing, China.
Qin DengDepartment of Clinical Nutrition, Children's Hospital of Chongqing Medical University, Chongqing, China.
Xionghui DingDepartment of Burn, Children's Hospital of Chongqing Medical University, Chongqing, China.
Chunbao GuoDepartment of Pediatrics, Chongqing Health Center for Women and Children, Chongqing, China.ORCID https://orcid.org/0000-0001-5121-9377

Funding

Chongqing Natural Science Foundation CSTB2022NSCQ-MSX0819Chongqing Natural Science Foundation cstc2019jcyj-msxmX0189Institute Research Program of Chongqing Health Center for Women and Children 2021YJQN03Scientific and Technological Research Program of Chongqing Municipal Education Commission KJZD-K202100406
6 · The paper itself

Abstract

This study investigates the role of Gasdermin D (GSDMD)-mediated pyroptosis in the pathogenesis of necrotizing enterocolitis (NEC) and reveals the mechanism by which reactive oxygen species (ROS) promote intestinal inflammation and injury through NLRP3/GSDMD pathway activation. Analysis of human NEC tissue samples showed significant oxidative stress (elevated MDA, decreased SOD activity, reduced GSH/GSSG ratio) and activation of the NLRP3/GSDMD/IL-1β pathway in the intestines of NEC patients. In an experimental NEC mouse model, GSDMD knockout (GSDMD-/-) significantly delayed NEC onset, reduced incidence, attenuated intestinal damage, and improved survival. GSDMD deficiency also decreased macrophage pyroptosis, systemic inflammation, and bacterial translocation, while improving intestinal barrier function. Pharmacological inhibition of GSDMD (using disulfiram) similarly mitigated pyroptosis and enhanced the bactericidal capacity of macrophages. Further mechanistic studies indicated that ROS drive pyroptosis by activating GSDMD, and the antioxidant N-acetyl-l-cysteine (NAC) could reverse this effect. The findings suggest that GSDMD is a potential therapeutic target for NEC, and strategies targeting GSDMD inhibition (genetic or pharmacological), combined with antioxidant therapy, may offer novel treatment approaches for NEC.

Indexed as

Enterocolitis, NecrotizingIntracellular Signaling Peptides and ProteinsNeoplasm ProteinsPhosphate-Binding ProteinsPyroptosisReactive Oxygen SpeciesAnimalsDisease Models, AnimalGasderminsHumansInfant, NewbornIntestinesMacrophagesMiceMice, Inbred C57BLMice, KnockoutGasderminsGSDMD protein, humanGsdmd protein, mouseIntracellular Signaling Peptides and ProteinsNeoplasm ProteinsNLR Family, Pyrin Domain-Containing 3 ProteinPhosphate-Binding ProteinsReactive Oxygen SpeciesdisulfiramGSDMDIL‐1βmacrophage pyroptosisNEC

Identifiers

PMID42689863
PMCPMC13539941

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.