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Objective: To systematically evaluate the Methods: Biodegradable FPU was synthesized using a two-step polymerization method. The hydrated particle size and the zeta potential of the micelles were measured by dynamic light scattering, and their morphology was observed by transmission electron microscopy. In accordance with the relevant standards for Results: The particle size of FPU micelles was approximately 132.5 nm, with a polydispersity index (PDI) of 0.096, which is below 0.150, indicating uniform particle size. The zeta potential of FPU micelles was －21.5 mV. After treatment with FPU micelles at various concentrations, the proliferation activity of NIH/3T3 and SKOV3 cells was not affected, and no significant toxicity was observed. Furthermore, cell membrane permeability was not significantly altered, and no significant apoptosis was induced. Cell cycle analysis showed that after 72 hours of treatment, the proportion of cells in the G1 phase increased, while the proportions of cells in the S and G2 phases decreased, suggesting potential G1 phase arrest. However, no significant toxicity was observed. Furthermore, hemocompatibility experiments demonstrated that FPU micelles did not cause erythrocyte rupture or hemolysis, further confirming their favorable biocompatibility. Conclusion: FPU micelles did not exhibit significant cytotoxicity or hemolysis