A dual‑site reactive ratiometric fluorescent probe for spermine and spermidine discrimination with visualizable sensing.

Qiye Liu, Yu Zhan, Pingping Sun, Anqi Li, Lian Xie, Qin Ding, Chong Chang, Shijun Chen, Chaoyuan Zeng, Weijie Chi

Abstract read

In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Qiye LiuSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China.

Yu ZhanSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China.

Pingping SunSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China. pingpingsun@hainanu.edu.cn.

Anqi LiSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China.

Lian XieSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China.

Qin DingSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China.

Chong ChangSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China.

Shijun ChenSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China. shijunchen@hainanu.edu.cn.

Chaoyuan ZengSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China. zengchaoyuan@hainanu.edu.cn.

Weijie ChiSchool of Chemistry and Chemical Engineering, State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, Hainan University, Haikou, 570228, China. weijie_chi@hainanu.edu.cn.ORCID https://orcid.org/0000-0003-1776-0025

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The bioamines spermine and spermidine, which are structurally similar and often coexist in complex matrices, still pose substantial challenges for achieving efficient detection of one another. To address this, a novel coumarin-based fluorescent probe, DX-Cl-CN has been developed. By introducing a chlorine atom and a vinylene-linked dicyanopropene motif as two dual reactive sites, the probe enables specific, differential responses toward spermine and spermidine across various bioamines. In DMSO, its maximum emission wavelength reaches 598 nm. Increasing solvent polarity induces a red shift in emission due to excited-state stabilization and enhanced molecular planarity. When interacting with spermine or spermidine, the probe exhibits a distinctive two-stage blue shift in fluorescence emission: its emission peak first shifts from 598 to 550 nm, then further to 486 nm. Ratiometric fluorescence analysis based on I

Indexed as

Biogenic aminesFluorescent probeNanofibrous filmRatiometric detectionSpermine and spermidine

Identifiers

PMID42687056

DOI10.1007/s00604-026-08365-0

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.