Evidence map›Paper›PMID 42687033›Full record

ArticleNature genetics2026

Spatiotemporal lineage tracing reveals the dynamic spatial architecture of tumor growth and metastasis.

Matthew G Jones, Dawei Sun, Kyung Hoi Joseph Min, William N Colgan, Haoyu Wang, Tivadar Török, Juliano Ribeiro, Jessie Xue, Erik C Cardoso, Yao Rong and 24 more

Abstract read
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In one paragraph

Article in Nature genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. The Duality of Collagens in Metastases of Solid Tumors.International journal of molecular sciences · 2025
    Review
  12. Review
  13. Article
  14. Review
  15. SpaceBar enables clone tracing in spatial transcriptomic data.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

34 authors.

Matthew G Jones *Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-0363-4493
Dawei Sun *Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.
Kyung Hoi Joseph MinDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-0894-4017
William N ColganDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-8328-4672
Haoyu WangDepartment of Systems Biology, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0001-9721-2891
Tivadar TörökBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0009-0007-6958-3557
Juliano RibeiroDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0009-0003-4511-4116
Jessie XueDepartment of Systems Biology, Columbia University, New York, NY, USA.
Erik C CardosoDevelopmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Yao RongDepartment of Systems Biology, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0002-1726-2555
Luyi TianBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-3420-3685
Jackson A WeirBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-8741-1344
Victor Z ChenDepartment of Systems Biology, Columbia University, New York, NY, USA.
Luke W KoblanDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Kathryn E YostDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nicolas Mathey-AndrewsDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Edridge D'SouzaDepartment of Medicine, Division of Hematology/Oncology, Columbia University Irving Medical Center, New York, NY, USA.ORCID http://orcid.org/0000-0003-4664-8163
Andrew J C RussellBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-5411-2807
Robert R StickelsBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.
Karol S BalderramaBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-8458-1364
William M RideoutDavid H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
Min DaiStanley Center for Psychiatric Research, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-7584-5014
Giovanni MarreroBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-1587-3624
Vipin KumarBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA.
Anjali SaqiDepartment of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, USA.
Benjamin HerzbergDepartment of Medicine, Division of Hematology/Oncology, Columbia University Irving Medical Center, New York, NY, USA.
Benjamin IzarDepartment of Systems Biology, Columbia University, New York, NY, USA.ORCID http://orcid.org/0000-0003-2379-6702
Howard Y ChangDepartment of Genetics, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-9459-4393
Joo-Hyeon LeeDevelopmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.ORCID http://orcid.org/0000-0002-7364-6422
Tyler JacksDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID http://orcid.org/0000-0001-5785-8911
Fei ChenBroad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA, USA. chenf@broadinstitute.org.ORCID http://orcid.org/0000-0003-2308-3649
Jonathan S WeissmanDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. weissman@wi.mit.edu.ORCID http://orcid.org/0000-0003-2445-670X
Nir YosefDepartment of Systems Immunology, Weizmann Institute of Science, Rehovot, Israel. nir.yosef@weizmann.ac.il.ORCID http://orcid.org/0000-0001-9004-1225
Dian YangDepartment of Systems Biology, Columbia University, New York, NY, USA. dy2491@cumc.columbia.edu.ORCID http://orcid.org/0000-0002-8455-0047

Funding

U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) 1DP2OD037078U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) 1K22CA289207U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI) 1K99CA286968
6 · The paper itself

Abstract

Tumor progression is driven by dynamic interactions between cancer cells and their surrounding microenvironment. Here we integrate high-resolution spatial transcriptomics and evolving lineage-tracing technologies to elucidate how tumor expansion, plasticity and metastasis co-evolve with microenvironmental remodeling in a Kras;Trp53-driven mouse model of lung adenocarcinoma. We find that subclonal expansion contributes to a hypoxic, immunosuppressive and fibrotic microenvironment that is associated with the emergence of prometastatic cancer cell states. We use tumor phylogeography to delineate intercellular interactions that are rewired in the expanding tumor niche and use co-culture systems to dissect how intercellular interactions and hypoxia influence cancer cell state. Furthermore, we find that metastases arise from spatially confined primary tumor subclones and remodel the distant metastatic niche into a fibrotic, collagen-rich microenvironment. Together, we present a comprehensive dataset integrating spatial assays and lineage tracing to elucidate how sequential changes in cancer cell state and microenvironmental structures cooperate to promote tumor progression.

Indexed as

AdenocarcinomaCell LineageLung NeoplasmsNeoplasm MetastasisTumor MicroenvironmentAnimalsCoculture TechniquesDisease ProgressionHumansMiceProto-Oncogene Proteins p21(ras)Spatial TranscriptomicsTumor Suppressor Protein p53Proto-Oncogene Proteins p21(ras)Tumor Suppressor Protein p53

Identifiers

PMID42687033

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.