Evidence map›Paper›PMID 42684913›Full record

Trial reportMicrobiology spectrum2026

Determination of diagnostic cycle threshold cutoffs for qPCR-based prevalence surveys of soil-transmitted helminth infections.

Malathi Manuel, Nils Pilotte, Joseph W S Timothy, Sean R Galagan, Gideon John Israel, Craig T Connors, Victor Omballa, Monica Pechanec Voss, Ushashi C Dadwal, Andrew M Gonzalez and 13 more

Abstract readClinical Trial, Phase IIIMulticenter StudyRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Malathi Manuel *The Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College Vellore, Vellore, India.ORCID 0000-0002-2575-9721
Nils Pilotte *Department of Biological Sciences, Quinnipiac University, Hamden, Connecticut, USA.ORCID 0000-0002-8447-7425
Joseph W S TimothyDepartment of Disease Control, London School of Hygiene & Tropical Medicine, London, United Kingdom.
Sean R GalaganDeWorm3 Project, University of Washington, Seattle, Washington, USA.
Gideon John IsraelThe Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College Vellore, Vellore, India.
Craig T ConnorsQuantigen, Fishers, Indiana, USA.
Victor OmballaDeWorm3 Project, University of Washington, Seattle, Washington, USA.
Monica Pechanec VossQuantigen, Fishers, Indiana, USA.
Ushashi C DadwalQuantigen, Fishers, Indiana, USA.
Andrew M GonzalezDepartment of Biological Sciences and Biochemistry, Smith College, Northampton, Massachusetts, USA.
Justin AhlonsouInstitut de Recherche Clinique du Bénin, Abomey Calavi, Bénin.
David ChaimaDepartment of Microbiology, Kamuzu University of Health Sciences (KUHeS) Blantyre, Blantyre, Malawi.
Zayina Zondervenni ManoharanThe Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College Vellore, Vellore, India.
Doug RainsQuantigen, Fishers, Indiana, USA.
Kristjana H ÁsbjörnsdóttirDeWorm3 Project, University of Washington, Seattle, Washington, USA.
Steve A WilliamsDepartment of Biological Sciences and Biochemistry, Smith College, Northampton, Massachusetts, USA.
Adrian J F LutyUniversité Paris Cité, IRD MERIT, Paris, France.
Moudachirou IbikounléInstitut de Recherche Clinique du Bénin, Abomey Calavi, Bénin.ORCID 0000-0002-3640-0175
Khumbo KaluaDepartment of Microbiology, Kamuzu University of Health Sciences (KUHeS) Blantyre, Blantyre, Malawi.
Robin L BaileyBlantyre Institute for Community Outreach, Lions Sight First Eye Hospital, Blantyre, Malawi.
Rachel L PullanDepartment of Disease Control, London School of Hygiene & Tropical Medicine, London, United Kingdom.ORCID 0000-0001-9844-1362
Judd L WalsonDeWorm3 Project, University of Washington, Seattle, Washington, USA.ORCID 0000-0003-4836-720X
Sitara S R AjjampurThe Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College Vellore, Vellore, India.ORCID 0000-0003-3419-6577

Funding

Bill and Melinda Gates Foundation OPP1129535
6 · The paper itself

Abstract

World Health Organization guidelines for control of soil-transmitted helminths (STHs) rely on coproscopic methods to assess population prevalence. In low-prevalence and light-intensity STH settings, quantitative PCR (qPCR) has higher sensitivity and specificity for detection. For qPCR to accurately identify transmissible infections of public health significance, it is essential to interpret qPCR cycle threshold (Ct) results. As part of the DeWorm3 community-based cluster-randomized trial on interrupting STH transmission, we conducted population-based surveys using high-throughput qPCR and aimed to establish appropriate Ct cutoffs to detect transmissible infections. Experimental approaches, including egg and genome-equivalent spiking experiments, were hindered by inefficient fecal DNA extraction despite optimization efforts. The Ct results for 29,980 samples (pre-intervention, cross-sectional surveys) revealed a bimodal distribution for two of the four species tested,

Indexed as

HelminthiasisHelminthsReal-Time Polymerase Chain ReactionSoilAnimalsAscariasisAscaris lumbricoidesCross-Sectional StudiesFecesHumansNecator americanusPrevalenceSensitivity and SpecificitySoilCt cutoffcycle thresholdqPCRsoil-transmitted helminths

Identifiers

PMID42684913
PMCPMC13689095

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.