ArticleSTAR protocols2026
Protocol for optogenetic control of cAMP in human telencephalic organoids using an easy-to-build Arduino-driven LED system.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Temporal regulation of cyclic adenosine monophosphate (cAMP) using photoactivated adenylyl cyclase (bPAC) is critical in research; however, commercial optogenetic systems are costly. Here, we present a protocol for optogenetic control of cAMP in human telencephalic organoids using an easy-to-build Arduino-driven light-emitting diode (LED) stimulation system. We describe steps for assembling the system, writing code for LED regulation, and preparing induced pluripotent stem (iPS) cells for optogenetic experiments. We then detail procedures for optogenetic activation of cAMP to control cell-fate decisions. For complete information on the generation and use of this protocol, please refer to Shimada et al.
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