Evidence map›Paper›PMID 42684466›Full record

ArticleMarine biotechnology (New York, N.Y.)2026

Functional Validation of the Phase I-II-III Detoxification Pathway in Azumapecten farreri using Optimized RNA Interference Technology.

Limin Lu, Guanchao Zheng, Yichen Lin, Chenfan Dong, Jixing Peng, Haiyan Wu, Zhijun Tan

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Limin LuCollege of Food Science and Engineering, Ocean University of China, Qingdao, 266404, China.
Guanchao ZhengKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Yichen LinKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Chenfan DongKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Jixing PengKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Haiyan WuKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Zhijun TanKey Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China. tanzj@ysfri.ac.cn.

Funding

Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences No. 2023TD76earmarked fund for CARS CARS-49National Natural Science Foundation of China No. 32472451
6 · The paper itself

Abstract

Paralytic shellfish toxins (PSTs) are widely distributed neurotoxins that accumulate and transform in bivalves, but the metabolic mechanisms after exposure of Azumapecten farreri (A. farreri) to PSTs remain unknown. To verify the phase I (cytochrome P450, CYP450)-II (glutathione S-transferase, GST)-III (ABC transport) metabolic genes for PSTs, we used RNA interference (RNAi) to silence target genes (CYP46A1, GSTM1, and ABCF2), measured PSTs content, and performed single nucleotide polymorphism (SNP) screening. Injection of small interfering RNA (siRNA) fragments into the scallop adductor muscle silenced target genes in multiple tissues. The three most effective fragments were selected. Secondary injection of screening fragments prolonged the silencing duration, with a silencing efficiency of 12.0%-100%. Silencing increased PSTs accumulation. Notably, with CYP46A1 silencing, the PSTs accumulation (1016 µg STXeq/kg) reached 3.85 times that of the control group. This silencing also delayed the conversion of highly toxic components (GTX1-4) to components with low toxicity (C1-2), thereby prolonging the PSTs metabolic process. SNP analyses indicated that PSTs exposure increased homozygous mutations in CYP49A1 (511908 site), this site is potentially involved in PSTs metabolism. In summary, this study optimized an RNAi method and confirmed the existence of a three-phase metabolic mechanism in scallops. CYP450 was found to be the main mode of PSTs metabolism. This study provides data regarding PSTs metabolic genes and loci in bivalves, as well as valuable molecular markers for anti-toxin breeding in shellfish.

Indexed as

Marine ToxinsPectinidaeRNA InterferenceAnimalsCytochrome P-450 Enzyme SystemGlutathione TransferasePolymorphism, Single NucleotideRNA, Small InterferingCytochrome P-450 Enzyme SystemGlutathione TransferaseMarine ToxinsRNA, Small InterferingAzumapecten farreriMetabolic mechanismParalytic shellfish toxinRNA interference (RNAi)Single nucleotide polymorphism (SNP)

Identifiers

PMID42684466

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.