ArticleJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology2026
The anti-coagulating agent heparin and native serum alter the barrier and tight junction properties of a primary cultured model fish gill epithelium.
Article in Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Primary cultured gill models are useful for investigating fish gill epithelium barrier properties. Typically, gill cell culture methods utilize commercially available heterologous serum supplements rather than native serum. This is because heterologous serum is easy to obtain and facilitates the development of models that mimic the gill very well. Also, native serum (or plasma) has historically had mixed success in primary cultured fish epithelium models. This study examined the effects of the blood anti-coagulating agent heparin and native serum on the barrier properties of a primary cultured trout (Oncorhynchus mykiss) gill model. Development of epithelia in the presence of heparin decreased transepithelial electrical resistance (TER) and increased paracellular movement of [
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