ArticlePlant cell reports2026
A stable transformation platform in pomegranate uncovers PgMYB10 as a key regulator of anthocyanin biosynthesis.
Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
key messageAn efficient genetic transformation platform enables functional validation of PgMYB10, identifying it as a master regulator governing anthocyanin biosynthesis in pomegranate. Limited availability of stable genetic transformation systems restricts functional genomics research in pomegranate. Here, we established efficient in vitro regeneration and Agrobacterium tumefaciens-mediated transformation systems for 'Taishanhong' pomegranate using stem segment explants. Optimized medium combinations produced high-frequency regeneration: a 93.3% shoot-induction rate on MS medium with 1.5 mg/L 6-benzylaminopurine (BAP), 0.6 mg/L 1-naphthaleneacetic acid (NAA) and 30.0 mg/L adenine sulfate (ADS); a proliferation coefficient of 5.4 on MS medium supplemented with 0.8 mg/L BAP and 0.3 mg/L indole‑3‑butyric acid (IBA); effective shoot-strengthening on MS medium containing 1.2 mg/L BAP, 0.3 mg/L NAA and 0.2 mg/L gibberellic acid (GA₃); and a rooting rate of 95.3% on half-strength MS medium with 1.5 mg/L IBA and 0.5 mg/L NAA. For transformation, precultured explants were immersed with A. tumefaciens suspension (OD₆₀₀ = 0.8) containing 20.0 mg/L acetosyringone (AS) for 30 min. After four days of dark co-culture, sequential antibiotic screening with 30 mg/L kanamycin and bacteriostatic treatment with 400 mg/L timentin yielded a stable average transformation efficiency of 17.5% in 'Taishanhong' pomegranate. Subsequent functional analysis revealed that overexpression of PgMYB10 induced pigment accumulation in leaves and stems. In three independent transgenic lines, maximum anthocyanin content and PgMYB10 transcript levels were 5.4-fold and 27.2-fold higher than in wild-type plants, respectively. Six anthocyanin biosynthetic genes (PgCHS, PgCHI, PgF3H, PgDFR, PgANS, and PgUFGT) were markedly upregulated, demonstrating that PgMYB10 positively controls anthocyanin biosynthesis. This transformation system provides a reliable technical platform for functional genomic studies in pomegranate, and PgMYB10 represents as a promising candidate gene for molecular breeding aimed at improving fruit pigmentation.
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