ArticleWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Assessment of Phenotype, Oxidative Stress and Biomacromolecule Oxidation in Human Chronic Skin Wound Epidermis in Active Debridement Tissue Versus Endstage Wounds.
Article in Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Impairment of cutaneous healing occurs in association with numerous comorbidities including diabetes, vascular insufficiency and immobility. Standard management of chronic wounds includes removal of necrotic tissue and infection management, but no biomarkers are used to guide appropriate debridement distance. We hypothesized that by understanding the spatial distribution of proteins associated with keratinocyte differentiation and activation, oxidative stress biomarkers, as well as accumulation of oxidative damage, debridement protocols could be refined. Human tissue was isolated from (1) routine debridement procedures or (2) endstage at limb amputation. In debridement samples, wound edge epithelium typically exhibited hyperproliferation with or without epibole, with endstage wounds exhibiting a much thinner epidermis. Cytokeratin 14 expression was increased throughout the suprabasal epidermis with cytokeratin 10 and filaggrin expression attenuated; endstage wounds displayed downregulation of both cytokeratin 10 and filaggrin further from the wound edge than observed in debridement tissue. Cytokeratin 6, 16 and 17 were expressed in the wound edge and adjacent uninjured epidermis in both debridement and endstage wounds, while cytokeratin 16 and 17 were expressed in the non-involved interfollicular epidermis in endstage limbs. Both phospho-nuclear factor kappa B and the antioxidant transcription factor phospho-nuclear factor-erythroid 2-related factor 2 show increased nuclear localization in the keratinocytes of endstage compared to debrided wounds concomitant with increased oxidative damage to lipids, protein and DNA in the epidermis. Our observations demonstrate increased tissue damage in endstage wounds compared to debridement tissue, which provides a preliminary starting point for the future investigation of several potential biomarkers associated with wound chronicity in humans.
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