ArticleMethods in molecular biology (Clifton, N.J.)2026
Simultaneous Visualization of NEAT1_2 and Paraspeckle Proteins by Sequential RNA-FISH and Immunofluorescence.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paraspeckles are membrane-less subnuclear assemblies whose organization depends on the long isoform of nuclear paraspeckle assembly transcript 1 (NEAT1_2), which scaffolds paraspeckle proteins into a core-shell architecture. Disruption of the layered structure within paraspeckles occurs when paraspeckle components are depleted, potentially leading to functional impairment. Here, we describe a protocol for simultaneous visualization of NEAT1_2 middle region and transactive response DNA-binding protein 43 (TDP-43), which are enriched in paraspeckle core and shell, respectively, in U2OS human osteosarcoma cells. Sequential RNA-fluorescence in situ hybridization (RNA-FISH) and immunofluorescence, combined with high-resolution confocal microscopy, enable delineation of the paraspeckle core-shell structure. This method provides a robust approach for probing paraspeckle remodeling across diverse biological conditions.
Indexed as
Identifiers
42681516What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.