ArticleMethods in molecular biology (Clifton, N.J.)2026
Labeling of Amine-Modified RNA.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
RNA labeling enables high-contrast visualization and quantitative measurements. This chapter describes practical guidance for fluorescent dye labeling of RNA via the NHS ester-amine coupling. We detail reaction setup, cleanup by ethanol precipitation, and evaluation of labeling efficiency by absorbance spectrum analysis or by high-performance liquid chromatography (HPLC). Notes summarize common failure modes and remedies, and RNase-free practice is emphasized throughout. Although the workflow demonstrates fluorescent labeling of 3'-amine-modified RNA, the protocol is broadly applicable to 5'-end or internal amine modifications and can also be used to introduce functional groups such as DBCO, used in click chemistry reactions.
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