ArticleMethods in molecular biology (Clifton, N.J.)2026
Determination of Bacterial Adherence to Mouse Tissues Using a Hematogenous Model.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This procedure is used to determine the tissue tropism of bacteria to mouse tissues. Briefly, a high dose of bacteria is inoculated intravenously via the mouse tail vein. Bacteria are allowed to circulate through the blood for a short duration of time, during which they will adhere to the endothelium in tissues for which there is a tropism. After the desired time, sterile saline is perfused through the animal and any unbound bacteria are washed away. Tissues harvested after perfusion will contain any bound bacteria, and tissue-specific tropism can be determined qualitatively by culturing live organisms from tissue or quantitatively by qPCR on DNA from harvested tissue. This method is also useful for determining bloodstream survival, a marker for complement resistance.
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