Evidence map›Paper›PMID 42681486›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Creating a Library of Random Transposon Mutants in Leptospira.

Christopher J Pappas, Hui Xu, Md A Motaleb

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Christopher J PappasDivision of Natural Sciences, Mathematics, and Computing, Manhattanville University, 2900 Purchase Street, Purchase, NY, 10577, USA. christopher.pappas@mville.edu.
Hui XuDepartment of Microbiology and Immunology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Greenville, NC, 27834, USA.
Md A MotalebDepartment of Microbiology and Immunology, Brody School of Medicine, East Carolina University, 600 Moye Boulevard, Greenville, NC, 27834, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Generation of a random transposon mutant library is advantageous in Leptospira as site-directed mutagenesis remains a challenge, especially in pathogenic species. This procedure is typically completed by transformation of Leptospira with a Himar1-containing plasmid via conjugation with Escherichia coli as a donor cell. Here, we describe the methodology to generate random transposon mutants in the saprophyte Leptospira biflexa via conjugation of plasmid pSW29T-TKS2 harbored in E. coli β2163. Determination of transposon insertion site by semi-random nested PCR will also be described. A similar methodology may be employed to generate Tn mutants of pathogenic Leptospira species.

Indexed as

DNA Transposable ElementsGene LibraryLeptospiraMutationConjugation, GeneticEscherichia coliMutagenesis, InsertionalMutagenesis, Site-DirectedPlasmidsPolymerase Chain ReactionDNA Transposable ElementsConjugationHimar1LeptospiraMutagenesisNested PCRSpirocheteTransposon library

Identifiers

PMID42681486

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.