Evidence map›Paper›PMID 42681479›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

EMEM and DMEM as an Alternative Media for Leptospiral Culture, Diagnostic, and Pathogenesis Studies.

Camila Hamond, Elsio A Wunder

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Camila HamondDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT, USA.
Elsio A WunderDepartment of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT, USA. elsio.wunder@uconn.edu.

Funding

Development of a Universal Vaccine Against LeptospirosisR01AI182354 · NIAID · UNIVERSITY OF CONNECTICUT STORRS · PI Elsio Augusto Wunder · 2024 to 2026
$2.3M
Evolutionary multispecies transcriptomics to reveal genes conferring pathogenicity within Leptospira sppR21AI163663 · NIAID · YALE UNIVERSITY · PI TOWNSEND, JEFFREY PETER, WUNDER, ELSIO AUGUSTO · 2021 to 2022
$461k
NIAID NIH HHS R01 AI182354NIAID NIH HHS R21 AI163663
6 · The paper itself

Abstract

Leptospires require specific conditions and nutrition to survive and thrive, essential for its ability to multiply or adapt to in vitro culture. This constraint on laboratory cultures limits the applicability of in vitro studies to the understanding of even simple pathogenic processes and diagnostics. Culture of leptospires in a laboratory setting using DMEM and EMEM media, common for culture of mammalian cells, is an alternative that mimics host environmental conditions, less complex, with high performance, and lower cost, which are imperative characteristics to improve routine laboratory activities and studies of leptospirosis.

Indexed as

Culture MediaLeptospiraLeptospirosisAnimalsBacteriological TechniquesHumansCulture MediaDiagnosticDMEMEMEMGrowth mediaLeptospiraLeptospiral pathogenesisMAT

Identifiers

PMID42681479

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.