Evidence map›Paper›PMID 42681461›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Protease-Free RNAscope and Sequential Immunofluorescence Methods for Manual Codetection of RNAs and Proteins.

John C Donaldson, Chengxin Zhou, Emerald Doolittle, Pino Bordignon, Cansaran Saygili Demir, Yifan Wang, Li-Chong Wang, Saska Brajkovic, Maithreyan Srinivasan, Henry Lamparski

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

John C DonaldsonAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA.
Chengxin ZhouAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA.
Emerald DoolittleAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA.
Pino BordignonLunaphore, A Bio-Techne brand, Rte de Lully 5c,, 1131, Tolochenaz, Switzerland.
Cansaran Saygili DemirLunaphore, A Bio-Techne brand, Rte de Lully 5c,, 1131, Tolochenaz, Switzerland.
Yifan WangAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA.
Li-Chong WangAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA.
Saska BrajkovicLunaphore, A Bio-Techne brand, Rte de Lully 5c,, 1131, Tolochenaz, Switzerland.
Maithreyan SrinivasanAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA.
Henry LamparskiAdvanced Cell Diagnostics, Inc., A Bio-Techne brand, Newark, CA, USA. Henry.Lamparski@bio-techne.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In situ hybridization (ISH) is an important method used in the visualization of gene expression/regulation signatures in cell and tissues. The ability to codetect mRNA and protein within the same tissue section on a single slide by combining proteomic immunohistochemistry (IHC) and transcriptomic ISH technologies has allowed for detailed spatial multiomic mapping within individual cells and their microenvironment. In this chapter, we describe the manual application of a universal permeabilization protease-free reagent, PretreatPro™, with RNAscope™ amplification and detection of mRNA in FFPE tissue samples for codetection of mRNA and protein without loss of antigenicity. PretreatPro is compatible with both chromogenic and fluorescent detection modalities when amplified with RNAscope.

Indexed as

Fluorescent Antibody TechniqueIn Situ HybridizationProteinsRNARNA, MessengerHumansImmunohistochemistryProteomicsTissue FixationProteinsRNARNA, MessengerCodetectionPretreatProProtease-freeRNAscopeSame sectionSpatial multiomics

Identifiers

PMID42681461

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.