High-Sensitivity In Situ Hybridization DNAscope for Detection of Papillomavirus or Epstein-Barr Virus Genomes Combined with Immunofluorescent Staining in Adherent Cells.

Malgorzata Bienkowska-Haba, Rona S Scott, Martin J Sapp, Katarzyna Zwolinska

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Malgorzata Bienkowska-HabaDepartment of Microbiology and Immunology, Center for Molecular and Tumor Virology, Center for Applied Immunology and Pathological Processes, Louisiana State University Health Sciences Center, Shreveport, LA, 71103, USA.ORCID http://orcid.org/0000-0002-9669-1875

Rona S ScottDepartment of Microbiology and Immunology, Center for Molecular and Tumor Virology, Center for Applied Immunology and Pathological Processes, Louisiana State University Health Sciences Center, Shreveport, LA, 71103, USA.ORCID http://orcid.org/0000-0003-3840-9189

Martin J SappDepartment of Microbiology and Immunology, Center for Molecular and Tumor Virology, Center for Applied Immunology and Pathological Processes, Louisiana State University Health Sciences Center, Shreveport, LA, 71103, USA.

Katarzyna ZwolinskaDepartment of Microbiology and Immunology, Center for Molecular and Tumor Virology, Center for Applied Immunology and Pathological Processes, Louisiana State University Health Sciences Center, Shreveport, LA, 71103, USA. katarzyna.zwolinska@lsuhs.edu.ORCID http://orcid.org/0000-0002-5872-7806

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hybridization methods have been broadly used for the observation of DNA of different origins. Herein, we describe the highly effective and specific in situ DNA hybridization protocol DNAscope for the identification of viral genomes in cells growing in monolayer cultures. The procedure utilizes the Z-probes and hybridization-based fluorescent detection systems available from Advanced Cell Diagnostics, ACD (Newark, CA, USA). We developed our protocol for the identification of episomal and integrated human papillomavirus (HPV) or Epstein-Barr virus (EBV) DNA in human keratinocytes. This procedure can be easily adapted to the detection of the wide range of DNA viruses in cell cultures or patient-derived samples. The protocol described here combines the detection of viral genomes by DNAscope with the visualization of the cellular protein expression with immunofluorescence staining (IF).

Indexed as

DNA, ViralFluorescent Antibody TechniqueGenome, ViralHerpesvirus 4, HumanHuman Papillomavirus VirusesIn Situ HybridizationPapillomaviridaeHumansKeratinocytesDNA, ViralCell cultureDNAscopeEpstein–Barr virus (EBV)HerpesvirusesHigh-sensitivity in situ hybridizationHuman papillomavirus (HPV)Immunofluorescence staining (IF)Viral genome detection

Identifiers

PMID42681459

DOI10.1371/journal.ppat.1006846

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.