ArticleMethods in molecular biology (Clifton, N.J.)2026
Use of Automated BaseScope Assay on the Leica BOND RX.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The in situ hybridization assay, BaseScope™, detects RNA transcripts in their spatial context, in settings in which enhanced detection is required. Such high sensitivity might be necessary because the target has: (a) low copy number of the target nucleic acid sequence in the cells of interest makes detection challenging with other methodologies, (b) a short target nucleic acid sequence (e.g., 50-300 nucleotides) limiting the number of probes that can be placed along it, (c) very closely related sequences from which it needs to be specifically discriminated. BaseScope™ enables the targeting of RNA sequences using as few as one to three pairs of 18-25 bp antisense oligonucleotide probes, combined with a highly sensitive chromogenic detection method. This assay can be performed on either the Leica BOND RX or the Ventana Discovery Ultra staining platforms; however, this chapter specifically focuses on the automated BaseScope™ assay using the Leica BOND RX platform.
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