Flow Cytometric Examination of Galectin Binding to Red Blood Cells.

Hau-Ming Jan, Hsien-Ya Lin, Dominique Paz, Vivien C Lee, Rebecca C Hale, Katelyn Sturgis, Carter J Stowell, Wenhui Li, Shang-Chuen Wu

Abstract read

PubMed Publisher

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hau-Ming JanJoint Program in Transfusion Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Hsien-Ya LinJoint Program in Transfusion Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Dominique PazDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.

Vivien C LeeJoint Program in Transfusion Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Rebecca C HaleDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.

Katelyn SturgisDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.

Carter J StowellDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.

Wenhui LiDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.

Shang-Chuen WuJoint Program in Transfusion Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. swu26@bwh.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lectins have the capacity to interact with cell surface carbohydrates, where they can influence various cellular processes. While early studies utilized lectin-induced cell agglutination to assess general binding characteristics, flow cytometry offers a more precise and quantitative method for measuring lectin interactions with specific cell populations. This technique is particularly valuable in studying galectins, a family of lectins that exhibit distinct binding preferences to red blood cells (RBCs) based on their blood type. This approach enables the determination of each lectin's binding specificity toward cell surface blood group antigens and other carbohydrate modifications without the potential confounding effects that differences in quaternary structure might have on their agglutination capabilities. We describe herein the production of fluorescently labeled galectins to analyze their specificity toward RBCs obtained from individuals with different blood types using flow cytometry.

Indexed as

ErythrocytesFlow CytometryGalectinsBlood Group AntigensFluorescent DyesHumansProtein BindingBlood Group AntigensFluorescent DyesGalectinsFlow cytometryFluorescence stainingGalectinRed blood cells

Identifiers

PMID42681448

DOI10.1007/978-1-0716-2055-7_11

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.