Evidence map›Paper›PMID 42681430›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Murine Models of Transfusion-Induced Red Blood Cell Alloimmunization.

Samata P Varadkar, Victoria Ortiz, Abraham B Alton, Brock D Hughes, Sasikala Muthusamy, Shang-Chuen Wu, William O Scott, Wenhui Li, Sean R Stowell, Connie M Arthur and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Samata P VaradkarDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Victoria OrtizDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Abraham B AltonDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Brock D HughesDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Sasikala MuthusamyDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Shang-Chuen WuDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
William O ScottDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Wenhui LiDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Sean R StowellDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Connie M ArthurDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Ryan P JajoskyDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA. rjajosky@bwh.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Blood transfusion, while a life-saving intervention, carries inherent risks ranging from infectious complications to non-infectious hazards, such as transfusion-related acute lung injury, circulatory overload, and hemolytic reactions. While donor screening and pathogen inactivation technologies can significantly reduce infectious risks, preventing non-infectious complications remains challenging due to limited understanding of donor and recipient factors that govern these outcomes. To address this, genetically tractable murine models can be employed to elucidate key biological factors governing immune-mediated complications of transfusion. However, for these models to yield meaningful results, experimental approaches must closely mirror clinical practice. We describe herein methods for obtaining, characterizing, and transfusing red blood cells in murine models, specifically focusing on studying alloimmunization.

Indexed as

ErythrocytesErythrocyte TransfusionTransfusion ReactionAnimalsDisease Models, AnimalHumansMiceAntibodiesIntraperitoneal injectionIntravenous injectionLateral tail veinMouse restrainerPassive immunizationRBC transfusionTail vein bleedingTail vein injection

Identifiers

PMID42681430

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.