Evidence map›Paper›PMID 42681419›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Analysis of Biotinylated Red Blood Cells Following Transfusion.

Mischa L Covington, Patricia E Zerra, Samata P Varadkar, Dominique Paz, Rebecca C Hale, Connie M Arthur, John D Roback, Ross M Fasano, Marianne E M Yee, Sean R Stowell

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mischa L CovingtonDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Patricia E ZerraDepartment of Pathology, Emory University School of Medicine, Atlanta, GA, USA.
Samata P VaradkarDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Dominique PazDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Rebecca C HaleDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
Connie M ArthurDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA.
John D RobackDepartment of Pathology, Emory University School of Medicine, Atlanta, GA, USA.
Ross M FasanoDepartment of Pathology, Emory University School of Medicine, Atlanta, GA, USA.
Marianne E M YeeDepartment of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA.
Sean R StowellDivision of Transfusion Medicine, Mass General Brigham, Harvard Medical School, Boston, MA, USA. srstowell@bwh.harvard.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Patients with sickle cell disease (SCD) can present with numerous complications, primarily associated with red blood cell (RBC) turnover, hemolysis, and associated immune pathway activation. While RBC transfusions can alleviate some of the most severe complications, the survival of transfused RBCs in chronically transfused patients varies significantly. To address this, novel protocols combining RBC biotinylation with flow cytometric detection have been developed, enabling more precise measurements of post-transfusion RBC survival. In an effort to elucidate the half-life of transfused RBCs in chronically transfused patients, cell-surface biotinylation has been integrated with Annexin V staining as a proof of concept. This approach allows for the examination of both RBC survival and changes in RBC lipid distribution, which often accompany cellular removal, providing valuable insights into the complex dynamics of RBC transfusions in patients with SCD.

Indexed as

Anemia, Sickle CellErythrocytesErythrocyte TransfusionFlow CytometryBiotinylationCell SurvivalHumansBiotinylated RBCs (BRBCs)Flow cytometryImmune pathwaysRBC lifespanRed blood cells (RBCs)Sickle cell anemiaTransfusion and blood storage

Identifiers

PMID42681419

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.