Evidence map›Paper›PMID 42681392›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Immunofluorescence Analysis of Epigenetic Markers in Oocytes and Preimplantation Embryos.

Marcella Pecora Milazzotto, Jessica Ispada

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Marcella Pecora MilazzottoLaboratory of Embryonic Metabolism and Epigenetics, Center of Natural and Human Science, Federal University of ABC, Santo Andre, Brazil. marcella.milazzotto@ufabc.edu.br.ORCID http://orcid.org/0000-0003-0933-3066
Jessica IspadaAnimal Biotechnology, AgResearch, Hamilton, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunofluorescence (IF) is an essential technique for studying epigenetic modifications in embryos. It offers high spatial resolution for precise visualization of chromatin-associated proteins and modifications involved in gene regulation and epigenetic reprogramming. IF allows multiplexing with multiple fluorophores, enabling the study of protein interactions and co-localization. Widely used in gametes and embryos across species, IF aids in analyzing epigenetic-related protein expression and localization during various stages of development.

Indexed as

BlastocystEpigenesis, GeneticFluorescent Antibody TechniqueOocytesAnimalsBiomarkersChromatinFemaleGene Expression Regulation, DevelopmentalHumansBiomarkersChromatinEmbryoEpigeneticGameteImmunofluorescence

Identifiers

PMID42681392

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.