ArticleMethods in molecular biology (Clifton, N.J.)2026
Analysis of mtDNA Methylation.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Whole-genome methyl sequencing facilitates the characterization of DNA methylation profiles within a reference genome. Recent identification of mitochondrial DNA (mtDNA) as an additional regulatory pathway within embryos paves the way for examining the impact of assisted reproductive technologies and other environmental factors on gamete and embryo developmental programming. Here, we describe the protocol for preparing libraries tailored to detect cytosine methylation in mtDNA extracted from minimal input, typical of experiments involving reproductive samples such as oocytes and embryos.
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