ArticleMethods in molecular biology (Clifton, N.J.)2026
Microwave-Assisted Dehydration, Non-Cryogenic Storage, and Rehydration of Spermatozoa and Germinal Vesicles.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The microwave-assisted dehydration technique enables preservation of both male and female genomes in a dried state at non-cryogenic temperatures, eliminating the reliance on liquid nitrogen for long-term storage. The dehydration strategy outlined below utilizes trehalose, a natural disaccharide known to ensure survival of certain primitive species under drought conditions, to increase the dehydration tolerance of the cells. In this chapter, we describe step-by-step procedures for trehalose loading, microwave-assisted dehydration, sample storage, and rehydration of both spermatozoa and germinal vesicle stage oocytes. Tips for modification are provided to apply the protocols to different species of interest.
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