Evidence map›Paper›PMID 42681378›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Sperm Proteomics and Phosphoproteomics: High-Throughput Methods Tailored to Gain Insight into Sperm Cell Signaling Pathways.

Elizabeth G Bromfield, Nathan D Burke, Shannon P Smyth, Georgia E Blackley, Jess E Mulhall, Brett Nixon, Sean J Humphrey, David A Skerrett-Byrne

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Special Issue "Advances in Spermatogenesis and Male Infertility".International journal of molecular sciences · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Elizabeth G BromfieldSchool of BioSciences, Faculty of Science, Bio21 Institute, The University of Melbourne, Parkville, VIC, Australia. liz.bromfield@unimelb.edu.au.ORCID http://orcid.org/0000-0001-7256-1403
Nathan D BurkeSchool of BioSciences, Faculty of Science, Bio21 Institute, The University of Melbourne, Parkville, VIC, Australia.
Shannon P SmythSchool of BioSciences, Faculty of Science, Bio21 Institute, The University of Melbourne, Parkville, VIC, Australia.
Georgia E BlackleySchool of BioSciences, Faculty of Science, Bio21 Institute, The University of Melbourne, Parkville, VIC, Australia.
Jess E MulhallInfertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton, NSW, Australia.
Brett NixonInfertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton, NSW, Australia.
Sean J HumphreyCharles Perkins Centre, School of Life and Environmental Sciences, University of Sydney, Sydney, NSW, Australia.
David A Skerrett-ByrneInfertility and Reproduction Research Program, Hunter Medical Research Institute, New Lambton, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein phosphorylation is critical to successful sperm cell maturation and oocyte interaction. However, despite longstanding and largely global use of protein tyrosine phosphorylation as a robust marker of "readiness for fertilization," a deeper understanding of sperm-specific phosphorylation events and phosphoproteins is still lacking. To address this critical knowledge gap, here we present a complete guide to produce and analyze highly comprehensive phosphoproteomes from human spermatozoa. This chapter provides a bespoke guide to in vitro human sperm capacitation, phosphopeptide enrichment, and high-throughput sample preparation for mass spectrometry based on the renowned EasyPhos method. Replete with hints for successful application, we intend this guide to be of equal use in improving our understanding of male infertility, as well as establishing new insight for the development of non-hormonal, sperm-targeted contraceptives.

Indexed as

PhosphoproteinsProteomeProteomicsSignal TransductionSpermatozoaHigh-Throughput Screening AssaysHumansMaleMass SpectrometryPhosphopeptidesPhosphorylationSperm CapacitationSperm ProteinsPhosphopeptidesPhosphoproteinsProteomeSperm ProteinsGerm cellsKinaseMass spectrometryPhosphatasePhosphoproteomePhosphorylationProteomicsSpermatogenesisSpermatozoaSperm capacitation

Identifiers

PMID42681378

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.