Evidence map›Paper›PMID 42681377›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Evaluation of Sperm Quality by Flow Cytometry: Sequential Protocols for DNA Fragmentation and Acrosome Reaction in Membrane-Intact Spermatozoa.

Sushmita Nath, Raul da Costa, Vesna Bojovic, Stefan Schlatt

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sushmita Nath *Centre of Reproductive Medicine and Andrology (CeRA), University Münster, Münster, Germany.
Raul da Costa *Centre of Reproductive Medicine and Andrology (CeRA), University Münster, Münster, Germany.
Vesna BojovicCentre of Reproductive Medicine and Andrology (CeRA), University Münster, Münster, Germany.
Stefan SchlattCentre of Reproductive Medicine and Andrology (CeRA), University Münster, Münster, Germany. Stefan.Schlatt@ukmuenster.de.ORCID http://orcid.org/0000-0002-7571-5313

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Evaluation of sperm DNA fragmentation and membrane integrity plays a critical role in understanding male fertility potential. Flow cytometry serves as a powerful and precise technique for this purpose. Our group has developed a convenient, reliable, and reproducible flow cytometry-based strategy for simultaneous evaluation of DNA and Membrane integrity (DMI). Building on this, we are currently conducting in vitro research on acrosome reaction analysis in live spermatozoa, an additional sperm membrane integrity-related parameter, using flow cytometry. This chapter presents both approaches as complementary protocols that can be run side by side: simultaneous evaluation of DNA and membrane integrity (DMI), and assessment of in vitro acrosome reaction and membrane integrity.

Indexed as

Acrosome ReactionCell MembraneDNA FragmentationFlow CytometrySemen AnalysisSpermatozoaAnimalsHumansMaleAcrosome reactionDMIDNA fragmentationFlow cytometryMembrane integritySperm

Identifiers

PMID42681377

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.