ArticleMethods in molecular biology (Clifton, N.J.)2026
Size Exclusion Chromatography with Multi-Angle Light Scattering for Detergent-Solubilized Integral Membrane Proteins.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Size-exclusion chromatography coupled to multi-angle static light scattering measures the shape-independent molar mass of macromolecules after chromatographic separation of different-sized species in a mixture. The use of a light-scattering detector together with two detectors that measure concentration by different methods allows the molar mass of detergent-solubilized integral membrane proteins to be analyzed in detail. The method determines the total molar mass of the protein-detergent complex, the molar mass of the protein component, and the molar mass of the detergent micelle. This information allows a user to assess the oligomeric state and polydispersity of the detergent-solubilized integral membrane protein before use in structural biology or functional studies.
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