Evidence map›Paper›PMID 42681287›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi.

Tatsuhiro Ezawa, Hayato Maruyama, Yusuke Kikuchi, Kaede Yokoyama, Chikara Masuta

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Tatsuhiro EzawaGraduate School of Agriculture, Hokkaido University, Sapporo, Japan. tatsu@agr.hokudai.ac.jp.
Hayato MaruyamaGraduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Yusuke KikuchiGraduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Kaede YokoyamaGraduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Chikara MasutaGraduate School of Agriculture, Hokkaido University, Sapporo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Downregulation of AM fungal genes using a plant viral vector is feasible. A partial sequence of a target gene is cloned into the multicloning site of the CMV2-A1 vector developed from RNA2 of Cucumber mosaic virus Y strain, and the RNA2, together with RNA1 and RNA3 of the virus, are in vitro-transcribed. Inoculation of Nicotiana benthamiana with these viral RNAs results in reconstitution of the virus in the plant, which triggers silencing of the fungal gene. Here, we describe not only the methods but also several tips for the successful application of virus-induced gene silencing to AM fungi.

Indexed as

CucumovirusGene SilencingMycorrhizaeGenetic VectorsNicotianaCucumber mosaic virusFungal geneNicotiana benthamianaVIGS

Identifiers

PMID42681287

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.