Evidence map›Paper›PMID 42681286›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Co-extraction of Small RNAs and Total RNA from Arbuscular Mycorrhizal Roots Using Laser Microdissection.

Valentina Fiorilli, Ignacio Rubio-Somoza, Raffaella Balestrini

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Valentina Fiorilli *Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy. valentina.fiorilli@unito.it.
Ignacio Rubio-Somoza *Molecular Reprogramming and Evolution (MoRE) Laboratory Centre for Research in Agricultural Genomics (CRAG), Barcelona, Spain.
Raffaella Balestrini *National Research Council of Italy, Institute of Biosciences and Bioresources (CNR-IBBR), Bari, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding gene expression at the cellular level is essential for dissecting plant-microbe interactions. Laser microdissection (LMD) is a powerful approach for isolating specific plant cell types without the need for molecular markers, enabling the recovery of nucleic acids from defined cellular populations. In plant systems, LMD has been widely applied to study cell-type-specific transcriptomes, including those involved in arbuscular mycorrhizal (AM) symbiosis, where cellular heterogeneity limits bulk tissue analyses. Recent evidence highlights the role of small RNAs (sRNAs) as key regulators of plant-microbe interactions, including cross-kingdom RNA interference. In this chapter, we describe a detailed LMD-based methodology for the co-extraction of sRNAs and mRNAs from AM-colonized root cells, enabling the characterization of plant and fungal sRNAs and the identification of their potential targets, thus providing a framework for studying cell-specific regulatory mechanisms in AM symbiosis.

Indexed as

Laser Capture MicrodissectionMycorrhizaePlant RootsRNA, FungalRNA, PlantRNA, MessengerSymbiosisRNA, FungalRNA, MessengerRNA, PlantAM fungiAM symbiosisCell-specificityGene expressionLaser microdissectionSmall RNATarget genesTotal RNA

Identifiers

PMID42681286

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.