ArticleMethods in molecular biology (Clifton, N.J.)2026
Collection of Arbuscular Mycorrhizal Hyphal Exudates Under in Vitro Culture Conditions.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Hyphal exudates of arbuscular mycorrhizal (AM) fungi can be collected in vitro using dual-compartment or split-plate systems. AM fungi are co-cultivated with host roots in one compartment, while hyphae grow across the central barrier into a root-free hyphal compartment. Once sufficient hyphal growth is established, sterile nutrient solution or phosphate-buffered saline solution is added to the hyphal compartment. After 24-48 h, the solution is collected. This method enables root-free access to AM fungal secretions for metabolic and functional analyses.
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